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Abstract
The Starlet Sea Anemone Nematostella vectensis (Nv) exists widely in coastal estuarine environments across North America. Nv has become a popular cnidarian model organism with a growing number of applications, including evolutionary developmental biology (EvoDevo), genomics, and cell biology. While previous research has established the ability of Nv to reproduce sexually and asexually, their reproductive behavior patterns have not been compared in any quantitative manner. As such, the purpose of this research is to understand how alterations in husbandry mechanisms, such as feeding schedule, aquarium substrate quality, temperature, and light exposure impact reproductive behavior of Nv and to establish trends for both modes of reproduction over time. Separate experiments are conducted to evaluate the aforementioned variables. Population data is collected over a period of five weeks and subsequently analyzed to determine reproductive impact. Additional experimentation is conducted utilizing singly-housed individuals under identical husbandry conditions, and the frequency of cloning and production are recorded over a two-month period. All experimental data is then analyzed using the appropriate statistical tests. Results of this analysis will pinpoint an optimal substrate quality, feeding frequency, temperature, and optimal light/dark period for both methods of reproduction. These results will also provide information regarding reproductive frequency and cycles in Nv maintained within a laboratory setting. The major aims of this body of work are to deepen the field’s understanding of reproductive behavior patterns within Nv, in an effort to optimize clonal generation for experiments, as well as improve quality of care for laboratory housed animals.