Corneal abrasions (CA) are one of the most common anesthesia-related adverse events. There is limited evidence about the effect of virtual education on anesthesia providers’ knowledge regarding CA prevention. The purpose of this study project was to assess if a web based educational module effectively improved anesthesia providers’ awareness of evidence-based preventative measures of CA. This was a quasi-experimental pretest-posttest study design. All participants completed a questionnaire regarding the knowledge on corneal abrasion prevention before and after the educational module. A student T-test was used to compare the differences in pre- and post- education knowledge scores. Among 26 participants, 15.4% were anesthesiologists, and 84.6% were CRNA. There was a significant pretest-posttest difference on the total knowledge score (6.54±1.14 vs. 8.55± 1.53, p< .001). Overall, the average number of correct answers increased from 6.54 ± 1.14 to 8.55 ± 1.53 (t = 5.74, p < .001) The web-based educational module was effective in the enhancement anesthesia providers’ knowledge in preventing surgical induced CA. The finding may add evidence on developing the effective guideline and standardizing provider education in CA prevention.