Dynamic exercise has been shown to improve glucose tolerance. However, it’s unclear if isometric exercise produces a similar benefit. The purpose of this study was to observe if an isometric exercise session acutely improved glucose tolerance in 16 apparently healthy males. Males, ages 18-45 yrs old, who participated in less than 90 minutes of weekly physical activity were recruited. During session 1, fasting blood glucose, baseline oral glucose tolerance, and bilateral maximum voluntary contraction (MVC) were determined. During session 2 (≥ 24 hours after session 1), fasting blood glucose was assessed followed by consumption for the glucose tolerance beverage. Immediately post consumption, subjects began six, 2-minute contractions at 15% MVC using bilateral quadriceps. Each contraction was separated by 1 minute of rest. Blood glucose was measured at 15, 30, 45, 60, 90, and 120 min post drink consumption. Results indicated that one session of isometric exercise did not alter glucose tolerance. Blood glucose was elevated immediately following exercise, which may be due to sympathoadrenal hyperactivity. Our findings suggest that one bout of isometric exercise is not an effective approach to acutely improve glucose tolerance. Future studies should employ isometric exercise training programs in Type II diabetic populations to further investigate potential improvements in glucose tolerance