BackgroundSugammadex is a useful reversal agent of neuromuscular blockade during surgery. However, its interaction with hormonal contraceptives could lead to undesirable outcomes in surgical patients using birth control pills. PACU nurses are responsible for patient education related to the interaction between Sugammadex and hormonal contraceptives. The feasibility and effectiveness of web-based education targeting post-anesthesia care unit (PACU) nurses is limited. Purpose The purpose of the project is to examine the effect of a web-based education on PACU nurses’ awareness and knowledge about the interaction between Sugammadex and hormonal contraceptives. Methods A quantitative, quasi-experimental, pre-/post-test design study was conducted among PACU nurses who work at a healthcare facility located in the southeast region of the United States. An online survey was used to investigate the effect of web-based education on PACU nurses’ knowledge about the interaction between Sugammadex and hormonal contraceptives. Results 27 PACU nurses were included in the study, their years of experience ranged from 0 to 26 years (4.43 ± 6.65). There was a significant pretest-posttest difference on Question 1 (the mechanism of action of Sugammadex) (χ 2 (1) = 6.22, p = .013). After the educational intervention, the average number of correct answers increased from 4.00 ± 0.87 to 4.70 ± 0.54 (t = 3.99, p < .001). Conclusion The web-based education was effective in improving PACU nurses’ knowledge of drug interactions. This finding may contribute to the development of a standardized online education program for PACU nurses, enhancing their skills and competence in providing patient education on anesthetic agents.