Malaria is a preventable disease that kills hundreds of thousands of people worldwide each year. Focusing on the sporozoite stage of the parasite Plasmodium berghei, we identified 561 differentially expressed genes (DEG) across different developmental stages using a datasetpublished in Nature, which contained salivary gland (SG) and midgut (MID) sporozoites. A recently published differential expression method PseudotimeDE was used for the DEG identification. We compared the DEG results with the reference paper, finding that PseudotimeDE identified fewer DEG than the current method, tradeSeq, for multiple possible reasons, and found several vaccine target genes. Finally, we ran Gene Ontology (GO) enrichment and identified additional potential malaria treatment genes, which will require future research to test and confirm.