The increasing prevalence of roads has had a corresponding impact on the risk of road mortality for wildlife, particularly for avian species such as birds of prey that commonly make use of foraging opportunities along roadside verges. Birds of prey, or raptors, are considered apex predators, which suggests that the impact of road mortality has far-reaching ecological consequences. The purpose of my research was to analyze the effect of traffic, habitat, and road verge variables on collision risk for raptors. I accomplished this in three ways: first, I assessed the impact of road and habitat variables on vehicle collision risk for Barred Owls (Strix varia) in the Charlotte, North Carolina region. I then applied this same analysis to both nocturnal and diurnal raptors in the Orlando, Florida region. Finally, I assessed the relative impact of species and individual traits on collision risk at locations with varying characteristics. Although I did not observe a difference in collision risk for raptors based on time of activity, I found that traffic volume and the suitability of surrounding habitat were significant predictors of collision risk in both Florida and North Carolina. In many cases, I found that increased prey cover in the form of brush, shrubs and tall grass along road verges served to predict collision risk in both locations. Complex vegetation provides habitat for small vertebrates, which in turn attracts raptors to roadsides, thus increasing the risk of being struck by a passing vehicle. My analysis of species traits showed that body size and reproductive output were the most important predictors of collision risk. Larger species and those with smaller clutch sizes were most likely to be hit, regardless of road conditions or habitat characteristics.