People living with HIV (PLWH) are among the most affected patient populations who suffer with SUDs. Health care providers (HCPs) must be knowledgeable about SUDs and cognizant of their own attitudes towards PLWH who suffer with substance abuse. Additionally, providers must be proactive in identifying HIV patients who are at risk for or actively engage in such high-risk behaviors, through appropriate screening. The purpose of this study was to examine the effects of a SUDs educational intervention on the knowledge, attitudes and screening frequency among HCPs who manage the care of PLWH. The study included pre- and post-test, and six weeks post trainig surveys. Data collection was done via retrospective chart reviews to assess the frequency of substance abuse screenings performed one year prior to the intervention, and again at three months before and after the training. The study sample consisted of 29 HCPs (medical doctors, fellows, pharmacists, nurse practitioners, a physician assistant, and a clinical social worker. Study results showed that there was a statistically significant increase in provider knowledge from the pre- to post-intervention (p = 0.000). There was no statistically significant difference six weeks post intervention (p = 0.080). Attitude scores were not statistically significant prior to, immediate post (p = 0.224) and six weeks post-intervention (p = 0.429). Providers did not show an increase in substance abuse screening frequency three months after the educational session. Additional HCP educational training is needed to sustain knowledge, improve attitudes, and increase frequency of screenings for substance use disorders among HIV patients.