Salt tolerance response revealed by RNA-Seq in a diploid halophytic wild relative of sweet potato

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Loraine, A., Luo, Y., Reid, R., & Song, B. -H. (2017). Salt tolerance response revealed by RNA-Seq in a diploid halophytic wild relative of sweet potato. Scientific Reports. https://doi.org/doi:10.1038/s41598-017-09241-x
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Faculty, Crop wild relatives harbor exotic and novel genetic resources, which hold great potential for crop improvement. Ipomoea imperati is a wild diploid relative of sweet potato with the capability of high salinity tolerance. We compared the transcriptomes of I. imperati under salt stress vs. control to identify candidate genes and pathways involved in salt response. De novo assembly produced 67,911 transcripts with a high depth of coverage. A total of 39,902 putative genes were assigned annotations, and 936 and 220 genes involved in salt response in roots and leaves, respectively. Functional analysis indicated a whole system response during salt stress in I. imperati, which included four metabolic processes: sensory initiation, transcriptional reprogramming, cellular protein component change, and cellular homeostasis regulation. We identified a number of candidate genes involved in the ABA signaling pathway, as well as transcription factors, transporters, antioxidant enzymes, and enzymes associated with metabolism of synthesis and catalysis. Furthermore, two membrane transporter genes, including vacuole cation/proton exchanger and inositol transporter, were considered to play important roles in salt tolerance. This study provided valuable information not only for understanding the genetic basis of ecological adaptation but also for future application in sweet potato and other crop improvements.

Details
Title

Salt tolerance response revealed by RNA-Seq in a diploid halophytic wild relative of sweet potato

Author(s)

Loraine, Ann
Luo, Yan
Reid, Robert
Song, Bao-Hua

UNC Charlotte Affiliate Type

Faculty

Extent

1 online resource

Date Created

2017

Genre

articles

Resource Type

text

Abstract

Crop wild relatives harbor exotic and novel genetic resources, which hold great potential for crop improvement. Ipomoea imperati is a wild diploid relative of sweet potato with the capability of high salinity tolerance. We compared the transcriptomes of I. imperati under salt stress vs. control to identify candidate genes and pathways involved in salt response. De novo assembly produced 67,911 transcripts with a high depth of coverage. A total of 39,902 putative genes were assigned annotations, and 936 and 220 genes involved in salt response in roots and leaves, respectively. Functional analysis indicated a whole system response during salt stress in I. imperati, which included four metabolic processes: sensory initiation, transcriptional reprogramming, cellular protein component change, and cellular homeostasis regulation. We identified a number of candidate genes involved in the ABA signaling pathway, as well as transcription factors, transporters, antioxidant enzymes, and enzymes associated with metabolism of synthesis and catalysis. Furthermore, two membrane transporter genes, including vacuole cation/proton exchanger and inositol transporter, were considered to play important roles in salt tolerance. This study provided valuable information not only for understanding the genetic basis of ecological adaptation but also for future application in sweet potato and other crop improvements.

Subjects--Topics

Genomics
Crop improvement

Is Part Of

Scientific reports

Language

English

Published Version (Please cite this version)

doi:10.1038/s41598-017-09241-x

Permalink

http://hdl.handle.net/20.500.13093/work:744

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