GSI treatment preserves protein synthesis in C2C12 myotubes

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Arthur, S., Huot, J., Marino, J. S., Thompson, B., & McMullen, C. (2021). GSI treatment preserves protein synthesis in C2C12 myotubes. Cells. https://doi.org/doi:10.3390/cells10071786
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Title

GSI treatment preserves protein synthesis in C2C12 myotubes

Author(s)

Arthur, Susan
Huot, Joshua
Marino, Joseph S.
Thompson, Brian
McMullen, Charlotte

UNC Charlotte Affiliate Type

Faculty

Publisher

MDPI

Extent

1 online resource

Date Published

2021

Material Type

Journal article

Genre

articles

Resource Type

text

Abstract

It has been demonstrated that inhibiting Notch signaling through γ-secretase inhibitor (GSI) treatment increases myogenesis, AKT/mTOR signaling, and muscle protein synthesis (MPS) in C2C12 myotubes. The purpose of this study was to determine if GSI-mediated effects on myogenesis and MPS are dependent on AKT/mTOR signaling. C2C12 cells were assessed for indices of myotube formation, anabolic signaling, and MPS following GSI treatment in combination with rapamycin and API-1, inhibitors of mTOR and AKT, respectively. GSI treatment increased several indices of myotube fusion and MPS in C2C12 myotubes. GSI-mediated effects on myotube formation and fusion were completely negated by treatment with rapamycin and API-1. Meanwhile, GSI treatment was able to rescue MPS in C2C12 myotubes exposed to rapamycin or rapamycin combined with API-1. Examination of protein expression revealed that GSI treatment was able to rescue pGSK3β Ser9 despite AKT inhibition by API-1. These findings demonstrate that GSI treatment is able to rescue MPS independent of AKT/mTOR signaling, possibly via GSK3β modulation.

Subjects--Topics

Muscle proteins
Cells

Is Part Of

Cells

Language

English

Published Version (Please cite this version)

doi:10.3390/cells10071786

Publisher's Version

https://www.mdpi.com/2073-4409/10/7/1786

Permalink

http://hdl.handle.net/20.500.13093/work:197

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