Transcriptome profiling of a beach-adapted wild legume for dissecting novel mechanisms of salinity tolerance

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Song, B. -H., Zhang, H., Zuelsdorf, C., & Penneys, D. (2018). Transcriptome profiling of a beach-adapted wild legume for dissecting novel mechanisms of salinity tolerance. Scientific Data. https://doi.org/doi:10.1038/sdata.2018.290
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Description

Strophostyles helvola is a close relative to common bean (Phaseolus vulgaris) and inhabits both coastal and non-coastal regions in North America. However, the mechanism of saline adaptation in S. helvola remains unclear. A transcriptome profiling would facilitate dissecting the underlying molecular mechanisms in salinity-adapted S. helvola. In this study, we reported the RNA-seq analyses of two genotypes (a salt-tolerant beach genotype and a salt-sensitive inland genotype) of S. helvola stressed with salt. S. helvola plants were grown in pots and treated with half lethal-guided dose of NaCl solution for 3 h, 24 h, and 7d. The plants supplied with the same amount of water were used as controls. The whole roots sampled from the three time points were equally pooled as one biological replicate, and three replicates were used for library construction and transcriptome sequencing on Illumina Hiseq 2500. The comparative analyses of root transcriptomes presented here provides a valuable resource for discovery of genes and networks involved in salt tolerance in S. helvola.

Details
Title

Transcriptome profiling of a beach-adapted wild legume for dissecting novel mechanisms of salinity tolerance

Author(s)

Song, Bao-Hua
Zhang, Hengyou
Zuelsdorf, Christine
Penneys, Darin

UNC Charlotte Affiliate Type

Faculty

Extent

1 online resource

Date Published

2018

Material Type

Journal article

Genre

articles

Resource Type

text

Abstract

Strophostyles helvola is a close relative to common bean (Phaseolus vulgaris) and inhabits both coastal and non-coastal regions in North America. However, the mechanism of saline adaptation in S. helvola remains unclear. A transcriptome profiling would facilitate dissecting the underlying molecular mechanisms in salinity-adapted S. helvola. In this study, we reported the RNA-seq analyses of two genotypes (a salt-tolerant beach genotype and a salt-sensitive inland genotype) of S. helvola stressed with salt. S. helvola plants were grown in pots and treated with half lethal-guided dose of NaCl solution for 3 h, 24 h, and 7d. The plants supplied with the same amount of water were used as controls. The whole roots sampled from the three time points were equally pooled as one biological replicate, and three replicates were used for library construction and transcriptome sequencing on Illumina Hiseq 2500. The comparative analyses of root transcriptomes presented here provides a valuable resource for discovery of genes and networks involved in salt tolerance in S. helvola.

Subjects--Topics

Plant ecology
Gene expression

Is Part Of

Scientific data

Volume

Vol. 5

Language

English

Published Version (Please cite this version)

doi:10.1038/sdata.2018.290

Permalink

http://hdl.handle.net/20.500.13093/work:178

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