SINGLE-STEP, REDUCED SHEAR, MICROFLUIDIC EXTRUSION OF BLOOD BRAIN BARRIER

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Wetzel, I. (2019). SINGLE-STEP, REDUCED SHEAR, MICROFLUIDIC EXTRUSION OF BLOOD BRAIN BARRIER. Unc Charlotte Electronic Theses And Dissertations.
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Abstract

Three-dimensional (3D) bioprinting of living structures with cell-laden biomaterials has been achieved in vitro, however, central nervous system tissues have been limited by the low viability printing of cerebral vessels, using conventional printing processes. To create a more viable cerebral vessel, reduced shear stress, multicellular, heterogeneous, perfusable blood brain barrier, a microfluidic print head and bioink was designed. Comsol simulations were used to map the theoretical flow profile of different microgroove structures to facilitate the design of a microfluidic print head. The print head was fabricated via standard SU-8 protocols and consisted of two A- symmetrically aligned PDMS pieces. To create a more viable and printable bio-ink, sodium alginate was mixed with highly concentrated collagen (35mg/ml) to form a cell friendly bio-ink. To confirm viability of the bioink and printing process, promodium iodide and nucleus dye were used on 2D disks. Endothelial cell attachment was observed 24 hours after plating. The 2D disk showed high viability of 97 ± 3% after 3 weeks of culture. In conclusion, microfluidic print head design simulation was capable of producing 3D hydrodynamic focusing of water. The bioink was capable of 2D printing process and producing a highly viable endothelial cell pallet. The microfluidic print head and bioink could be used in unison for future bio printing systems to quickly print vessels with smaller size or have the addition of the different cell types to create a neurovascular unit.

Details
Author

Wetzel, Isaac

Title

SINGLE-STEP, REDUCED SHEAR, MICROFLUIDIC EXTRUSION OF BLOOD BRAIN BARRIER

Physical Description

1 online resource (36 pages) : PDF

Date

2019

Degree Granting Institution

University of North Carolina at Charlotte

Abstract

Three-dimensional (3D) bioprinting of living structures with cell-laden biomaterials has been achieved in vitro, however, central nervous system tissues have been limited by the low viability printing of cerebral vessels, using conventional printing processes. To create a more viable cerebral vessel, reduced shear stress, multicellular, heterogeneous, perfusable blood brain barrier, a microfluidic print head and bioink was designed. Comsol simulations were used to map the theoretical flow profile of different microgroove structures to facilitate the design of a microfluidic print head. The print head was fabricated via standard SU-8 protocols and consisted of two A- symmetrically aligned PDMS pieces. To create a more viable and printable bio-ink, sodium alginate was mixed with highly concentrated collagen (35mg/ml) to form a cell friendly bio-ink. To confirm viability of the bioink and printing process, promodium iodide and nucleus dye were used on 2D disks. Endothelial cell attachment was observed 24 hours after plating. The 2D disk showed high viability of 97 ± 3% after 3 weeks of culture. In conclusion, microfluidic print head design simulation was capable of producing 3D hydrodynamic focusing of water. The bioink was capable of 2D printing process and producing a highly viable endothelial cell pallet. The microfluidic print head and bioink could be used in unison for future bio printing systems to quickly print vessels with smaller size or have the addition of the different cell types to create a neurovascular unit.

Genre

masters theses

Subjects--Topics

Biomedical engineering

Degree

M.S.

Keywords

Barrier
Bioprinting
Blood
Brain
Hydrogel
Microfluidic

Subject Area

Mechanical Engineering

Advisor(s)

Cho, Hansang

Committee Members

Cho, Hansang
Clemens, Mark
Lee, Charles
Dreau, Didier
Marriot, Ian

Degree Note

Thesis (M.S.)--University of North Carolina at Charlotte, 2019.

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Rights Holder Information

Copyright is held by the author unless otherwise indicated.

Identifier

Wetzel_uncc_0694N_12092

Permalink

http://hdl.handle.net/20.500.13093/etd:2535